BREEDING SCIENCE

METHODS OF INSEMINATION

IN-SEASON brood

Most greyhound broodes come into season every nine months, although some will cycle every six months.  The season usually lasts for twenty-one days, and this should be counted from the first day she shows color, a blood stained discharge.  When a brood comes into season, they will usually pay more attention to their rear-end, and her vulva will become swollen.  Also, frequent urination is a tell-tale sign.

 

PROGESTERONE TESTING

Through extremely accurate methods of progesterone testing, one can ascertain exactly the correct day for mating, to ensure the highest likelihood for successs. Previous theories and some existing sire masters believe the timing for breeding the female between the 12th and 15th day, however, scientific sireies have proven that this can actually be anything from the 5th to the 23rd day. If the brood is not mated at exactly the correct time it is very likely she will miss or have a small litter.

THE BENEFITS OF FROZEN SEMEN

International Canine Semen Bank - Full Document- Website -

 Reproductive technology has taken great strides in recent years as has the acceptance of giving mother nature a hand in reproducing quality fancy dogs. Many breeders today are using artificial insemination technology in their breeding programs for a number of good reasons. Fresh insemination processes require both dog and brood to be present or insemination to occur within 48 hours of obtaining the semen. More and more breeders and veterinarians are moving to a frozen semen process that allows breeding to take place conveniently and safely. It is important to understand the significant advantages that artificial insemination and the use of frozen semen have for many breeders over the traditional natural breeding method.

The process is simple, yet very effective and takes up to an hour for collection and storage. Semen to be frozen is collected using a teaser brood or smell, and standard collection procedures. A nutrient medium, which keeps the semen alive, is added and then it is frozen and stored in liquid nitrogen. ICSB has developed a proprietary freezing process that is both cost effective and highly successful. The ICSB method does less damage to the sperm during freezing than traditional freezing methods due to a larger surface area and quicker freezing time. The ICSB method of sperm storage provides the highest rate of pregnancy because it delivers more viable sperm for insemination. It has a significantly higher rate of pregnancies than natural mating practices. The semen can be stored in liquid nitrogen at the ICSB labs indefinitely. When needed, the semen is easily shipped to the broodes. local veterinarian who can inseminate the brood and have her back to her home the same day. 

 

The benefits of artificial insemination include:

 

SURGICAL IMPLANT

Internet Animal Hospital - Website

From start to finish, the surgical Artificial Insemination procedure takes only a few minutes.   Light general anesthesia is required.  Absolute sterile procedure is an important factor.  The insemination fluid must be kept warm and implanted within the uterus as soon as possible after collection from a male who is present, or soon after shipped, frozen semen is thawed. The Procdure is as follows:

Incision is made in the midline abdomen

ARTIFICIAL INSEMINATION

Internet Animal Hospital - Website

The semen and associated nutrient fluids are infused directly into the deep vaginal area of the female.   The female then will have her rear quarters raised for a few minutes in an attempt to have gravity assist with the dispersal of the semen as deep into the vaginal area and as close to the cervical opening as possible.  Then the female needs to remain confined for 6 to 8 hours after the insemination. The individual sperm cells need to pass through the cervix from the vagina and into the body of the uterus.  From there, they disperse further up the two "Horns" of the uterus.  The uterine horns are long hollow tubes, lined by the richly vascular endometrium, and extend from the body of the uterus upward almost to each kidney.  At the end of these two tubes the ovaries are situated such that when they produce eggs, the eggs travel through a tiny opening into the far end of each uterine horn.   If the eggs encounter sperm cells in their travels DOWN the uterine horns, there is a chance that the egg will be fertilized by some lucky sperm cell going UP the uterine canal and then another whole series of miracles take place that just might end up as a puppy.

 

TRANS CERVICAL INSEMINATION

This new procedure allows semen to be deposited directly into the cervix without anesthesia.  Special training is needed with the trans cervical technique in order to be certain that the semen is deposited within the uterus.  The procedure is performed  using an endoscope.

 

NGA DNA PROGRAM

Kansas State University CVM - Dr. Brad Fenwick- Full Document- Website-

The NGA DNA Program has been phased in so that DNA samples from all sires and dams used for breeding are stored by the NGA. The DNA Repository at Kansas State University now holds samples for over 7,500 Greyhounds. Breeding Greyhounds were phased into the program as follows:

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